pr8b00883_si_002.xlsx (60.99 MB)
Robust, Reproducible, and Economical Phosphopeptide Enrichment Using Calcium Titanate
dataset
posted on 2018-12-21, 00:00 authored by Adnan Ahmed, Vijay J. Raja, Paola Cavaliere, Noah DephoureMass-spectrometry-based
phosphoproteomics has revolutionized phosphoprotein
analysis and enhanced our understanding of diverse and fundamental
cellular processes important for human health and disease. Because
of their relative scarcity, phosphopeptides must be enriched before
analysis. Many different enrichment methods and materials have been
described, and many reports have made claims about the advantages
of particular materials and methodological variations. We demonstrate
an effective and highly reproducible single-step enrichment method
using an off-the-shelf preparation of calcium titanate. Using two
different cell lines and replicate analysis, we show that our method
achieves a purity and depth of analysis comparable or superior to
a widely used TiO2-based method at a reduced cost and effort.
This method provides a new and immediately available tool for expanding
the reach of phosphoproteomic inquiry.