Retromer-associated actin regulators colocalize with CCV actin patches.

(A and B) CCV actin patches colocalize with Arp3, cortactin, WASH, and FAM21. The retromer protein VPS35 also colocalizes with patches whereas N-WASP does not. Vero cells at 3 dpi were immunostained for the indicated proteins. Histogram depicts the means ± SD of ≥ 60 cells for at least 3 independent experiments. Statistical significance was determined using Student’s t-test (****P <0.0001). Colocalization was determined using Pearson’s correlation coefficient. NMII, C. burnetii Nine Mile phase II strain. Scale bar, 5 μm.