Retrograde RABV infection is unaffected by axonal interferon treatment.

(A) Cell bodies in S at 24 h post RABV P-mCherry infection in N. IFNβ or IFNγ was added to N for 24 h prior to axonal infection (scale bars = 250 μm). (B) Quantification of % infected cell bodies at 24 hpi (+/-) IFNβ or IFNγ in N or S. Black dots represent individual tri-chambers (from three independent replicates). Horizontal lines and errors bars represent mean ± SD for each condition with *p = 0.0114, ****p < 0.0001 using one- way ANOVA (ns = not significant).