Retention time for lipid mediators panel under different UHPLC-MS/MS chromatography conditions (pdf)

The chromatography parameters differ by mobile phase pH (3.8, 5.8 and 6.4). Using a elution method of UHPLC with a reversed-phase column (C18), a mixture of commercially lipid mediators standards elutes according to their polarity, number of double bonds and chain length, allowing the separation of most isomeric and isobaric species (represented by blue and red lines respectively) dependent of pH and according to identifying fragmentation patterns. An asterisk represented the non-detect metabolites.