Results of RT-QuIC analysis.

<p>ThT fluorescence was measured by relative fluorescence units (rfu) with saturation occurring at 65,000 for every 42 min. The RT-QuIC responses were performed using 10<sup>−3</sup> dilution of sCJD patient with 129MM type brain homogenate (NHBX0/0001), 10<sup>−5</sup> CJD negative control brain homogenate (NHBZ0/0005), 15ul of 10<sup>−1</sup> dilution of CSF from 4 gCJD cases with V180I (the Patients nos. 1, 2, 3 and 5) and 1 sCJD case with 129MM. Each point represents the mean of 4 replicate rfu readings.</p>