Representative histologic staining with safranin O–fast green (×100) of the ankle joint of mice with collagen-induced arthritis (CIA) mice after daily IFN-β therapy for 7 days

<p><b>Copyright information:</b></p><p>Taken from "Treatment with recombinant interferon-β reduces inflammation and slows cartilage destruction in the collagen-induced arthritis model of rheumatoid arthritis"</p><p>Arthritis Research & Therapy 2004;6(3):R239-R249.</p><p>Published online 23 Mar 2004</p><p>PMCID:PMC416442.</p><p>Copyright © 2004 van Holten et al., licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.</p> (Upper panel) In CIA mice treated with saline as controls, hardly any safranin O staining was observed in the ankle joints. (Lower panel) In CIA mice treated daily with 2.5 μg IFN-β, significantly less loss of safranin O staining was observed, indicating inhibition of cartilage breakdown. Histologic analysis of cartilage in CIA in mice after 7 days of IFN-β therapy. Hind paw sections were stained with safranin O–fast green, which stains the cartilage proteoglycans. Sections were scored in a blinded manner on a 4-point scale as described in Materials and methods. Significantly less loss of safranin O staining was observed in the animals treated with IFN 2.5 μg than in controls (= 0.03), indicating inhibition of cartilage destruction. *Statistically significant difference.