Representative flow cytometry gating strategy.

Alveolar cells were surface stained for CD45, CD4, CCR6 and then intracellularly stained for IL-17A and Interferon-gamma (IFN-ɣ). We gated on single cells, CD45+ cells, live CD4+ T cells, and then for either CCR6+IL17A+ cells or total IFN-ɣ producing cells. SSH: Side Scatter Height, SSA: Side Scatter Area.