Gb-2007-8-3-r32-2.jpg (44.29 kB)
Representative AD-associated alternative splicing event identified using splice array technology
figure
posted on 2011-12-31, 06:45 authored by Erin L Heinzen, Woohyun Yoon, Michael E Weale, Arjune Sen, Nicholas W Wood, James R Burke, Kathleen A Welsh-Bohmer, Christine M Hulette, Sanjay M Sisodiya, David B GoldsteinCopyright information:
Taken from "Alternative ion channel splicing in mesial temporal lobe epilepsy and Alzheimer's disease"
http://genomebiology.com/2007/8/3/R32
Genome Biology 2007;8(3):R32-R32.
Published online 7 Mar 2007
PMCID:PMC1868939.
Schematic of alternative splicing event associated with mTLE. Exons are shown in orange and intronic regions are shown in gray. Data collected for the alternative splice event in control and AD brain tissue (TC and CB) samples using the splice array technology (left) and quantitative rtPCR (qrtPCR, right). Data are presented as mean ± standard error of the mean; *< 0.05 when compared to control, Student's -test. rtPCR confirmation of the pattern of transcript expression in brain tissue collected from ten subjects from each group. Reference and variant transcript forms were amplified using the following primer sequences (indicated in the figure by arrows above mRNA transcripts): reference GABRA6F-AAGAATCTTCAAGCCTTCTCCA, GABRA6R-TGACAGCTGCGAACTCGATA, variant GABRA6F-AAGAATCTTCAAGCCTTCTCCA, GABRA6F-TCCAAGATTACACAAATCTTTATATGC.