Reduction in the recruitment of M2 macrophages in wound granulation tissues by a COX-2 inhibitor.

(A–D) Real-time PCR analyses of the expression levels of the IL-1β (A), iNOS (B), MR (C), and Ang-1(D) mRNAs in day 3 granulation tissues from mice treated with or without celecoxib (100 mg/kg). Data are expressed as the mean ± SEM (n = 6). *P < 0.05. (E, F) The left panels show representative images of CD11b (green) (E, F) and IL-1β (red) (E) or MR (red) (F) in immunostained day 3 wound granulation tissues from mice treated with or without celecoxib (100 mg/kg). Scale bars: 10 μm. The data are quantified in the right panel. Data are expressed as the mean ± SEM (n = 6). ***P < 0.001.