ac401613x_si_002.zip (5.4 MB)
Real-Time Metabolomics on Living Microorganisms Using Ambient Electrospray Ionization Flow-Probe
dataset
posted on 2013-08-06, 00:00 authored by Cheng-Chih Hsu, Mariam
S. ElNaggar, Yao Peng, Jinshu Fang, Laura M. Sanchez, Samantha J. Mascuch, Kirsten A. Møller, Emad K. Alazzeh, Jiri Pikula, Robert A. Quinn, Yi Zeng, Benjamin E. Wolfe, Rachel J. Dutton, Lena Gerwick, Lixin Zhang, Xueting Liu, Maria Månsson, Pieter C. DorresteinMicroorganisms
such as bacteria and fungi produce a variety of
specialized metabolites that are invaluable for agriculture, biological
research, and drug discovery. However, the screening of microbial
metabolic output is usually a time-intensive task. Here, we utilize
a liquid microjunction surface sampling probe for electrospray ionization-mass
spectrometry to extract and ionize metabolite mixtures directly from
living microbial colonies grown on soft nutrient agar in Petri-dishes
without any sample pretreatment. To demonstrate the robustness of
the method, this technique was applied to observe the metabolic output
of more than 30 microorganisms, including yeast, filamentous fungi,
pathogens, and marine-derived bacteria, that were collected worldwide.
Diverse natural products produced from different microbes, including Streptomyces coelicolor, Bacillus
subtilis, and Pseudomonas aeruginosa are further characterized.
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Streptomyces coelicolortechniquemicrobeagriculturebacteriamicrojunction surface sampling probesample pretreatmentIonizationvarietyMetabolomicresearchDiversescreeningLiving Microorganismsdrug discoveryBacillus subtilismethodpathogenyeastElectrosprayPseudomonas aeruginosataskionize metabolite mixturesrobustnessagarfilamentous fungiAmbientspectrometryelectrospray30 microorganisms
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