Reactivity of sorbose dehydrogenase from <i>Sinorhizobium</i> sp. 97507 for 1,5-anhydro-d-glucitol

<div><p>Purified recombinant sorbose dehydrogenase from <i>Sinorhizobium</i> sp. 97507 exhibited high reactivity for 1,5-anhydro-d-glucitol (1,5-AG) and l-sorbose, but little activity for the other sugars or sugar alcohols tested. Kinetic analysis revealed that its catalytic efficiency (<i>k</i><sub>cat</sub><i>/K</i><sub>m</sub>) for l-sorbose and 1,5-AG is 1.8 × 10<sup>2</sup> and 1.5 × 10<sup>2</sup> s<sup>−1</sup>·M<sup>−1</sup>, respectively.</p></div>