Quantification of sex differences in ultrasonic vocal signals emitted by adult male and female mice.

Cumulative density and box plots represent individual vocal features for all signals emitted by either males (blue) or females (orange). Analyses combined data from each recorded dyadic interaction. For box plots, the vertical black line indicates the median value. The left and right edge of each box represents the 25^th and 75^th percentile, respectively. The vocal features examined were bandwidth (A), duration (B), high frequency (C), low frequency (D), mean frequency (E), slope (F), and amplitude (G). Statistical analyses were conducted using Mann-Whitney U-tests. * = p < 0.05, ** = p < 0.01.