Proteomic and molecular analyses define GlCHC interacting proteins.

(A) Venn diagram indicating 36 specific hits for the GlCHC derived co-IP and 62 hits present in both co-IP datasets. (B-I) IFA validation of HA-tagged candidate GlCHC interacting partners: aside from GlDRP (F), all proteins exhibit exclusive localization in the cell cortex. Cells were imaged at maximum width, where nuclei and the bare-zone are at maximum diameter. Representative images for HA-tagged Gl15411(B), Gl4259 (C), Glα-adaptin(D), Glβ-adaptin(E), Gl16653(G), Gl7723(H) and Gl16595(I). (J) GlCHC-coIP based interactome validated by subcellular localization of reporter constructs. Each GlCHC-interacting protein depicted in (J) was used in reverse coIP analyses (K) to further validate the interactions. Full and dashed lines indicate high and low interaction stringency parameters, respectively.