pr3007254_si_004.pdf (328.29 kB)
Proteomic and Bioinformatic Profile of Primary Human Oral Epithelial Cells
journal contribution
posted on 2012-11-02, 00:00 authored by Santosh K. Ghosh, Elizabeth Yohannes, Gurkan Bebek, Aaron Weinberg, Bin Jiang, Belinda Willard, Mark R. Chance, Michael T. Kinter, Thomas S. McCormickWounding of the oral mucosa occurs frequently in a highly
septic environment. Remarkably, these wounds heal quickly and the
oral cavity, for the most part, remains healthy. Deciphering the normal
human oral epithelial cell (NHOEC) proteome is critical for understanding
the mechanism(s) of protection elicited when the mucosal barrier is
intact, as well as when it is breached. Combining 2D gel electrophoresis
with shotgun proteomics resulted in identification of 1662 NHOEC proteins.
Proteome annotations were performed based on protein classes, molecular
functions, disease association and membership in canonical and metabolic
signaling pathways. Comparing the NHOEC proteome with a database of
innate immunity-relevant interactions (InnateDB) identified 64 common
proteins associated with innate immunity. Comparison with published
salivary proteomes revealed that 738/1662 NHOEC proteins were common,
suggesting that significant numbers of salivary proteins are of epithelial
origin. Gene ontology analysis showed similarities in the distributions
of NHOEC and saliva proteomes with regard to biological processes,
and molecular functions. We also assessed the interindividual variability
of the NHOEC proteome and observed it to be comparable with other
primary cells. The baseline proteome described in this study should
serve as a resource for proteome studies of the oral mucosa, especially
in relation to disease processes.