DIY GFP beads 08-2018.pdf (570.19 kB)
Production of magnetic GFP affinity beads
presentation
posted on 2018-08-02, 12:53 authored by Nichanok Auevechanichkul, Ruth Lintermann, Lennart WirthmuellerLennart WirthmuellerOur protocol for production of 'home-made' magnetic GFP affinity beads using a Camelid a-GFP nanobody. These can be used to immuno-purify GFP- or YFP-tagged proteins from cell extracts.
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DFG grant WI 3670/2-1 and CRC973
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- Biochemistry and cell biology not elsewhere classified
- Plant biology not elsewhere classified
- Zoology not elsewhere classified
- Animal cell and molecular biology
- Bioinformatic methods development
- Bioinformatics and computational biology not elsewhere classified
- Other biological sciences not elsewhere classified
- Plant cell and molecular biology
Keywords
GFPYFPCo-immunoprecipitation (co-IP)immuno-precipitatedpulldown experimentsaffinity resinprotein purification protocolsNanobody ConjugatesdiyGFP fusion proteinBiochemistry and Cell Biology not elsewhere classifiedPlant BiologyZoologyAnimal Cell and Molecular BiologyBiological TechniquesCell BiologyMolecular Biology
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