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Procaspase 8 inducing drugs in acute in vitro HIV infection model.
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posted on 2017-06-19, 17:37 authored by Rahul Sampath, Nathan W. Cummins, Sekar Natesampillai, Gary D. Bren, Thomas D. Chung, Jason Baker, Keith Henry, Amélie Pagliuzza, Andrew D. BadleyA) Primary uninfected CD4 cells from 3 donors were infected with HIV IIIB and then incubated with procaspase 8 inducing drugs for 48hours with RAL and TDF. ATP, HIV DNA by qPCR (B), and supernatant P24 (D) were assessed. C) The degree of procaspase 8 expression induction by ELISA was compared between drugs which reduced HIV DNA or did not reduce HIV DNA in panel (B). Depicted are means (SD). E) Pearson correlation coefficient between the change in cell associated HIV DNA and change in supernatant p24 was assessed.
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vivo patient cells HIVCD 4 T cellscytotoxic protein Casp 8pprocaspase 8 levelscell-associated HIV DNA levelsHIV reactivationincrease Casp 8p9 drugsPrimary CD 4 T cellscell-associated HIV DNAHIV protease cleaving procaspase 8.procaspase 8 expressionCasp 8p levelsHIV IIIb infection modelHIV DNA levelsScreening 1277 FDAcell deathreactivating cells
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