Pro- and anti-inflammatory cytokines´ expression was induced after mice treatment with rTcHMGB.

Total RNA was extracted from mice spleens at 3, 6 and 24 h post inoculation with rTcHMGB, rGST, LPS or vehicle (Physiological solution), and relative cytokines´ mRNA levels were determined by qRT-PCR using RPLP0 as housekeeping gene. Data are expressed as the fold change of the control non-treated group (white column) and presented as Mean ± SEM; * p<0.05. Cytokines analyzed were (A) TNF-α, (B) IL-1β, (C) IFN-γ, (D) TGF-β, (E) IL-10.