Primer sets used for amplification of the Clonorchis sinensis cytochrome c oxidase subunit 1 (cox1) gene by the loop-mediated isothermal amplification (LAMP) technique.
(A) Locations of the primer sequences. (B) Names, length and sequences of six primers. F3 and B3 represent forward and backward external primers, respectively; FIP and BIP represent forward and backward internal primers, respectively; and LF and LB represent forward and backward loop primers, respectively. Primer FIP consists of F1 complementary sequence and F2 direct sequence. Primer BIP consists of B1 direct sequence and B2 complementary sequence.