Preparation of EHE-CM for mass spectrometry.

<p>Representative images of chicken heart explant at 0 hour (A), EHE co-culture at 24 hours (B) and 36 hours (C). Scale bar: 80 μm in (A); 40 μm in (B-C). (D) Silver-stained SDS-PAGE gel of 3 independent preparations, CM1-3. (E) Western blot of TGFβ2 and TGFβ3 in CM preparations. CM, conditioned medium; H, heart explant; HL, heart lysate; A, atria; V, ventricle.</p>