pone.0166940.g002.tif (1.02 MB)
Predicted miR-125b downstream target genes in PE placentas.
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posted on 2016-12-09, 18:56 authored by Weiwei Yang, Anning Wang, Chunling Zhao, Qinghua Li, Zhifang Pan, Xuefu Han, Cuijuan Zhang, Guohui Wang, Chao Ji, Guili Wang, Guangtao Jia, Jiyu Ju, Wei Gao, Wenjing Yu, Xiaoying Liu, Xi Chen, Weiguo Feng, Zhiqin Gao, Jie Li, Chune RenThe mRNA levels of ZNF543 (A), IRF4 (B), SGPL1 (C) and HIF1AN (D) was detected using Real-time qPCR in placentas derived from PE patients and the controls. Protein expression of SGPL1 is reduced in both basal (E) and chorionic plates (F) preeclamptic placentas. (G) Immunohistochemistry assay was performed in paraffin sections of human placenta to show the localization of sphingosine-1-phosphate lyase 1 (SGPL1), arrows indicate trophoblast cells. Results are presented as mean ± SEM. Statistical comparison between miR-125b mimics (or inhibitor) and the corresponding NC was performed using SPSS, with p<0.05 considered as significant. *p < 0.05, **p < 0.01.
