Plasma processing method comparison.

Whole blood was collected from 20 NSCLC patients and processed using various tube types, incubation times and centrifugation steps. A subset of these patients (n = 5) had whole blood processed to assess a single vs. double centrifugation step. DNA was measured using the ABI TaqMan® RNase P Detection Reagent Kit A: Blood was collected using EDTA blood collection tube (EDTA) and processed after 2 hr or 72 hr. B: Blood was collected using cell free DNA blood collection tube (cfDNA BCT) and processed after 2 hr or 72 hr. C: Blood was collected using EDTA or cfDNA BCT tubes and processed after 2 hr. D: Blood was collected using EDTA or cfDNA BCT tubes and processed after 72 hr. E: Blood collected in EDTA tubes after 2 hr incubation and processed using either single or double centrifugation. F: Blood collected in EDTA tubes after 72 hr incubation and processed using either single or double centrifugation. Results are displayed for each patient. Statistical analysis was performed using a paired Student’s t-test where; **p<0.01.