Phytochemical, Antioxidant, Antimicrobial, and Protein Binding Qualities of Hydro-ethanolic Extract of Tinospora cordifolia

The study was performed to check the chemical, antioxidant, antimicrobial and protein binding qualities of the hydro-ethanolic extract of Tinospora cordifolia (TC). In qualitative and quantitative analysis of extract, the major compounds were tinocordioside, cordifolide A, palmatine, quercetin, β-sitosterol, heptacosanol, and syringin. Hydro-ethanolic extract of TC exhibited low antibacterial and antifungal activities. Excellent antioxidant activities (60–80 %) were noticed for various antioxidant assays viz DPPH (2,2-diphenyl-1-picrylhydrazyl), MC (Metal chelating), FRAP (Ferric reducing), SA (Superoxide radical), and NO (Nitric oxide). Total phenolic content (TPC) and total flavanoids content (TFC) of the extract was 2.38±0.15 mg/g (expressed in equivalents to gallic acid) and 18.91±0.21 mg (expressed in equivalents quercetin) respectively. Moreover, at p < 0.05, significant linear correlation was noticed between the TFC/TPC and antioxidant activities favoured which highlights the medicinal property of TC. Protein binding assay was performed and an observed binding constant (1.03±0.92×10^-5 μM^-1) was almost close to control (aspirin). Anti-inflammatory assay revealed potent anti-inflammatory activity of hydro-alcoholic extract of TC. These significant findings of TC may provide compelling scientific evidence of its therapeutic potential.