Phase-contrast and fluorescence microscopy of P. aeruginosa PAO1 in water.

A) Mid-log cells of P. aeruginosa under phase contrast. B) P. aeruginosa incubated in water for 12 days under phase contrast. C) LIVE/DEAD staining results of P. aeruginosa PAO1 following incubation in water for 1, and D) 12 weeks. Cells were grown to mid-log in LB, washed, and added to sdH₂O at a concentration of 10⁷ CFU/ml and incubated at room temperature. Cells were added to agarose beds on glass slides and visualized on a Leica microscope.