Fig 2.TIF (1.41 MB)

Patients with TB have less B-cells.

figure

posted on 2016-06-15, 03:30 authored by Simone A. Joosten, Krista E. van Meijgaarden, Franca del Nonno, Andrea Baiocchini, Linda Petrone, Valentina Vanini, Hermelijn H. Smits, Fabrizio Palmieri, Delia Goletti, Tom H. M. Ottenhoff

PBMCs from individuals infected with Mtb (LTBI), with active TB disease or successfully treated TB patients and controls were thawed and stained directly for several combinations of B-cell surface markers. Lines indicate the median values of all groups. Ethnicity of donors was indicated using the following symbols: ‘black circle’ = West Europe; ‘black diamond’ = Est Europe; ‘black square’ = Africa; ‘black triangle’ = Asia; ‘black star’ = Sud America. Groups were compared to the uninfected controls using the Mann-Whitney test and a p< 0.05 was considered significant. * marks differences that remained significant after multiple test correction using Kruskal-Wallis testing with Dunn’s post-test. A. The percentage of CD19⁺ total B-cells in the different patients group expressed within the total lymphocyte population; CD20^-CD21^- plasma cells expressed as percentage of CD19⁺ B-cells; CD10⁺ immature B-cells as percentage of CD19⁺ B-cells and IgD⁺CD27^- naïve B-cells as percentage of total CD19⁺ B-cells. The analysis was performed in 28 controls, 22 LTBI individuals, 22 TB patients and 27 TB treated subjects, except for plasma cells which were enumerated in 16, 14, 16, 13 individuals respectively. B. Regulatory B-cell populations were enumerated within the total CD19⁺ B-cells for a subset of individuals (11 control, 8 LTBI, 9 TB, 11 TB treated) by surface staining of B-cells within PBMCs.