Fig 4.TIF (966.5 kB)
Patch-clamp investigation of the optochemokine tandem in cell attached configuration at 34°C.
figure
posted on 2016-10-21, 17:37 authored by Katrin Feldbauer, Jan Schlegel, Juliane Weissbecker, Frank Sauer, Phillip G. Wood, Ernst Bamberg, Ulrich TerpitzThe pipette solution was supplemented with 50 nM SDF1α. a. Typical current trace recorded at an applied membrane potential of -100 mV. Light-dependent signal of tCXCR4/CatCh directly after the sealing process and 30 min later. During that time the cell was illuminated every two minutes for 100 ms. b. Time course of the relative tCXCR4/CatCh current in presence of SDF1α (square, 5 cells) or SDF1α and the inhibitor AMD3100 (triangle, 3 cells), and CatCh in presence of SDF1α (circle, 4 cells). Mean values are given, bars represent the standard error.
History
Usage metrics
Categories
Keywords
tandem endosomescytosollight-induced releasedyes rhod 2shuttle proteinplasma membraneOptochemokine TandemGPCRrhod 2-AMrhodopsin tandeminternalization kineticsintracellular areaschemokine receptor CXCR 4CatCh signalconfocal laser scanning microscopypatch-clamp measurementsoptochemokine tandemtCXCRSDF
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC