PU.1 knockdown cancels GM-CSF-mediated increase of mRNAs.

A-D. Quantitative real-time PCR analysis of the mRNA expression of PU.1 (A), HLA-DRα (B), total CIITA (C), and CIITA driven by pIII (D) in PU.1 siRNA (PU.1)- or control siRNA (cont.)-introduced CAL-1 cells. Cells were incubated for 24h after siRNA transfection and cultured for additional 72h with GM-CSF stimulation. The results are expressed as means ± SEMs of three independent experiments. Each experiment was performed with duplicate samples. *, p < 0.05.