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PU.1 knockdown cancels GM-CSF-mediated increase of mRNAs.

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posted on 2016-04-22, 06:26 authored by Ryosuke Miura, Kazumi Kasakura, Nobuhiro Nakano, Mutsuko Hara, Keiko Maeda, Ko Okumura, Hideoki Ogawa, Takuya Yashiro, Chiharu Nishiyama

A-D. Quantitative real-time PCR analysis of the mRNA expression of PU.1 (A), HLA-DRα (B), total CIITA (C), and CIITA driven by pIII (D) in PU.1 siRNA (PU.1)- or control siRNA (cont.)-introduced CAL-1 cells. Cells were incubated for 24h after siRNA transfection and cultured for additional 72h with GM-CSF stimulation. The results are expressed as means ± SEMs of three independent experiments. Each experiment was performed with duplicate samples. *, p < 0.05.

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