Fig 4.tif (1.06 MB)

PBMCs stimulated with cross-reactive microbial peptides can suppress HIV-1 infection.

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posted on 2018-02-21, 19:52 authored by Christopher W. Pohlmeyer, Sarah B. Laskey, Sarah E. Beck, Daniel C. Xu, Adam A. Capoferri, Caroline C. Garliss, Megan E. May, Alison Livingston, Walt Lichmira, Richard D. Moore, M. Sue Leffell, Nicholas J. Butler, Jennifer E. Thorne, John A. Flynn, Robert F. Siliciano, Joel N. Blankson

(A-B) Results of PBMC suppression assay. PBMCs from HIV-1-positive HLA-B*27⁺ subjects were stimulated for five days with no peptide, CEF pooled peptides, KK10 peptide, or KK10CR peptide and then infected with highly concentrated IIIB virus. After 40 hours, infection of CD3⁺/CD8^- cells was measured by staining for intracellular HIV-1 Gag. Values below 20% (in grey) fall below the limit of quantification. Part A shows flow cytometric results for subject ES31; part B shows summarized results for five subjects with CEF pooled peptides, KK10 peptide, and individual KK10CR peptides (C-D) PBMCs from HIV-1-positive HLA-A2⁺ subjects were stimulated for five days with no peptide, CEF pooled peptides, SL9 peptide, or SL9CR peptide and then infected with highly concentrated IIIB virus. After 40 hours, infection of CD3⁺/CD8^- cells was measured by staining for intracellular HIV-1 Gag. Part C shows flow cytometric results for subject CP37; part D shows summarized results for seven subjects with CEF pooled peptides, SL9 peptide, and individual SL9CR peptides.