Overexpression of Mal3 (1–143) exhibits the TBZ-tolerance in the wild type strain.
(A) Wild type (PR109) strains harboring pREP3X (vector), pREP3X-mal3 (1–308; full-length), pREP3X-mal3 (1–143), or pREP3X-mal3 (135–308) were cultured as described in Fig 1C. Cells were streaked on EMMU in the presence or absence of 40 μg/mL TBZ or 60 μg/mL TBZ. All plates were incubated for 5 days at 30˚C. (B) S. cerevisiae AH109 strain was co-transformed with pGBKT7-derived plasmid and pGAD424-derived plasmid and selected on SC lacking leucine and tryptophan (SC-LW). Transformed cells were cultured and the cell suppression was spotted as described in Fig 2B. All plates were incubated for 3 days at 30˚C.