Osteoprogenitor cells can enhance early bone formation in critical bone defects in dogs

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posted on 2017-11-29, 11:27 authored by Endrigo Gabellini Leonel Alves, Rogéria Serakides, Isabel Rodrigues Rosado, Omar Leonardo Aristizabal Paez, Jéssica Alejandra Castro Varon, Felipe Nemer Machado, Fabíola Bono Fukushima, Alfredo Miranda Góes, Cleuza Maria de Faria Rezende

ABSTRACT: The aim of this study was to evaluate the effect of osteoprogenitor cells derived from mesenchymal stem cells from adipose tissue (OC-AD-MSCs), and differentiated into osteoblasts, in the treatment of critical bone defects in dogs. Adipose tissue derived mesenchymal stem cells (AD-MSCs) were subjected to osteogenic differentiation for 21 days and used in the treatment of bone defects in dogs radius. Either three experimental groups were bone defects treated with OC-AD-MSCs (OC), defects filled with autogenous bone (Control- C +), or empty defects (Control- C -). Bone regeneration was assessed by radiology, densitometry, and histomorphometry. The area of new bone formation was higher in the OC group compared to the control group (C-) on postoperative day 15. Defects treated with OC-AD-MSCs showed greater neovascularization than the other two groups at 90 days. We concluded that treatment with OC-AD-MSCs increased the area of new bone formation 15 days after surgery; however, it didn’t complete the bone union in critical bone defects in the radius of dogs at 90 days.