Organic Carbon Plays an Important Role in Antarctic Lake Region Soil Bacterial Diversity

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posted on 2018-12-04, 11:20 authored by Wenbing HanWenbing Han, Nengfei Wang, Yue Ma, Jinjiang Lv, Shuang Wang, Botao Zhang, Zhihui Jiang, Huansheng Cao

16S rRNA gene amplicons were sequenced on an Illumina MiSeq platform, and 250-bp paired-end reads were generated. Clean reads were obtained by removing barcode and primer sequences, trimming end bases and filtering low-quality bases, with QIIME (version 1.7.0) (Caporaso et al. 2010b), chimeras were detected by the UCHIME algorithm (Edgar Robert C. et al. 2011) and against the Gold database, and finally the chimeric sequences and lllumina adapters were removed

Funding

This research was supported by National Key R&D Program of China (2018YFC1406706), National Natural Science Foundation of China (41776198), and Development Fund of Marine Bioactive Substances, SOA (MBSMAT-2017-01)

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Keywords

Bacterial Community Composition MotivationEstimation geochemical factor, high-throughput sequencing organic carbon Antarctic Genetics Microbial Genetics

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