Fig 4.TIF (3.65 MB)
Nuclear staining of Capan-1 cells following 48 h treatment with HMNE3.
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posted on 2016-10-19, 17:50 authored by Yong-Chao Ma, Zhi-Xin Wang, Shao-Ju Jin, Yan-Xin Zhang, Guo-Qiang Hu, Dong-Tao Cui, Jiang-Shuan Wang, Min Wang, Fu-Qing Wang, Zhi-Jun ZhaoApproximately 5×103 cells/mL were seeded on 35-mm glass slides. After treatment with HMNE3 for 48 h, the cells were washed twice with PBS and incubated with 5 μg/mL Hoechst 33258 for 10 min at 37°C in the dark. The nuclear morphology was then examined under a fluorescent microscope.
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HMNE 3TUNELHRPtopoisomerase II activityreceptor tyrosine kinasetyrosine kinaseHMNE 3 dosestumor cell proliferationterminal deoxynucleotidyl transferasetopoisomerase II proteinsRTKDNA plasmid pBR 322agarose gel electrophoresisHoechst 33258 fluorescence staining3.2 μ M HMNE 3Human Pancreatic Cancer Cellsnovel bis-fluoroquinolone chalcone-liketopoisomerase IIIC 50 valuecancer cell linesNovel Bis-Fluoroquinolone Chalcone-Liketyrosine kinase activityMTT
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