bm6b00098_si_001.pdf (60.98 kB)
Neuronal Differentiation of Induced Pluripotent Stem Cells on Surfactant Templated Chitosan Hydrogels
journal contribution
posted on 2016-03-23, 00:00 authored by Kristan
S. Worthington, Brian
J. Green, Mary Rethwisch, Luke A. Wiley, Budd A. Tucker, C. Allan Guymon, Aliasger K. SalemThe development of effective tissue
engineering materials requires
careful consideration of several properties beyond biocompatibility,
including permeability and mechanical stiffness. While surfactant
templating has been used for over a decade to control the physical
properties of photopolymer materials, the potential benefit of this
technique with regard to biomaterials has yet to be fully explored.
Herein we demonstrate that surfactant templating can be used to tune
the water uptake and compressive modulus of photo-cross-linked chitosan
hydrogels. Interestingly, templating with quaternary ammonium surfactants
also hedges against property fluctuations that occur with changing
pH. Further, we demonstrate that, after adequate surfactant removal,
these materials are nontoxic, support the attachment of induced pluripotent
stem cells and facilitate stem cell differentiation to neuronal phenotypes.
These results demonstrate the utility of surfactant templating for
optimizing the properties of biomaterials intended for a variety of
applications, including retinal regeneration.