Multiple genomic locations of 2 SM gene clusters.

(A) SM gene cluster 1 (Afu1g00970-01010) and flanking region are found in different genomic locations. The flanking regions contain transposon-derived open reading frames, including 2 putative reverse transcriptases. In one strain, SM gene cluster 1 is found adjacent to SM gene cluster 30. (B) Synteny of A. fumigatus SM gene cluster 1 with clusters in Phaeosphaeria nodorum, Pseudogymnoascus pannorum, Escovopsis weberi, and Hypoxylon sp. CI4A. All species contain nonsyntenic genes predicted by antiSMASH to be part of a biosynthetic gene cluster. (C) SM gene cluster 33 (Afu5g00100-00135) is found in different genomic locations in different strains. In one strain, the cluster is adjacent to SM gene cluster 34. Multiple transposable elements flank the cluster in each strain. EOS, end of scaffold; FAD, flavin adenine dinucleotide; MFS, major facilitator superfamily; ORF, open reading frame; PKS, polyketide synthase; SM, secondary metabolite.