Morphology of established hypothalamus-derived cell lines.

<p>a, b. Representative images of established hypothalamus-derived cell lines. c, d. Representative images of electron microscopy. A few secretory granules (c: arrow) and intermediate filaments (d: dotted circle) were observed. e-g. Cells (11–55) were stained with anti-AgRP and anti αMSH antibodies (e: AgRP, f: αMSH, g: merge). h. Western blot analysis after Tricine SDS page of the culture medium of the cell lines and AgRP control peptide (50 pmol) with anti-AgRP antibody.</p>