Fig 2.tif (8.55 MB)
Morphology of established hypothalamus-derived cell lines.
figure
posted on 2016-02-05, 19:36 authored by Hiroshi Iwakura, Katsuko Dote, Mika Bando, Hiroyuki Koyama, Kiminori Hosoda, Kenji Kangawa, Kazuwa Nakaoa, b. Representative images of established hypothalamus-derived cell lines. c, d. Representative images of electron microscopy. A few secretory granules (c: arrow) and intermediate filaments (d: dotted circle) were observed. e-g. Cells (11–55) were stained with anti-AgRP and anti αMSH antibodies (e: AgRP, f: αMSH, g: merge). h. Western blot analysis after Tricine SDS page of the culture medium of the cell lines and AgRP control peptide (50 pmol) with anti-AgRP antibody.
