Morphological evaluation of vitrified testicular tissue from prepubertal domestic cats.

<p>(A) proportion of intact seminiferous tubules, (B) proportion of Sertoli cells stained with vimentin in each seminiferous tubule, (C) connexin expression relative to pixel intensity. Data are expressed as mean ± SE (n = 6 animals per treatment). Different letters above bars indicate significant statistical differences between treatments (P < 0.05). Warming 1 (tissue warmed at 37°C), culture 1 (warming 1 followed by culture for 24h), warming 2 (tissue warmed at 50°C), culture 2 (warming 2 followed by culture for 24h).</p>