AR-3-2-118-7.jpg (46.42 kB)
Monocyte migration induced by supernatants of RA SCLs stimulated with TNF-α
figure
posted on 2011-12-31, 04:13 authored by Kenji Hayashida, Toshihiro Nanki, Hermann Girschick, Sule Yavuz, Takahiro Ochi, Peter E LipskyCopyright information:
Taken from "Synovial stromal cells from rheumatoid arthritis patients attract monocytes by producing MCP-1 and IL-8"
Arthritis Research 2001;3(2):118-126.
Published online 5 Jan 2001
PMCID:PMC17828.
Copyright © 2001 BioMed Central Ltd on behalf of the copyright holder
Assay of monocyte migration was performed using supernatants of RA SCLs stimulated with TNF-α. Each RA SCL (1 × 10/well) was cultured in six-well culture plates with 2 ml DMEM supplemented with 5% FBS with or without 2 ng/ml TNF-α. Supernatants were collected after 24 h of incubation, and were used for assay. Migrated cells were stained with anti-CD14 mAb, then the number of migrated CD14 cells was assessed by flow cytometry. RA6/1, RA8/3, and Sy77 are RA SCLs. The mean and SEM were calculated from three independent experiments. Statistical analysis was performed with the paired Student test. < 0.05 and < 0.01.