1471-2180-3-2-1.jpg (36.57 kB)
Molecular cloning and characterization of K12 gene-0
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posted on 2011-12-30, 17:18 authored by Natalya N Samsonova, Sergey V Smirnov, Irina B Altman, Leonid R PtitsynCopyright information:
Taken from "Molecular cloning and characterization of K12 gene"
BMC Microbiology 2003;3():2-2.
Published online 31 Jan 2003
PMCID:PMC150594.
Copyright © 2003 Samsonova et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
G gene (b3073) with aer-ygjG intergenic region, and sites for restriction enzymes used in cloning procedure are shown. The orientation of genes and location of σpromoter are represented by arrows. Nucleotides numbering is in relation to mRNA transcription initiation site designated as +1. upstream fragments fused with and subcloned into pBRP [] vector are shown as bold lines. B. The sequence of upstream region. Nucleotides numbering as in part A. σpromoter, putative NRand IHF binding sites, SD sequences and the first mRNA base are bold. The consensus sequences for IHF, NRand σpromoter are shown above the main sequence. Two possible translation start codons are underlined and corresponding N-terminal amino acids are bold. The putative FIS binding site is overlined. The directions of ORF1 and ORF2 translation are shown by arrows.