MoEnd3 is involved in endocytosis and F-actin assembly.

(A) Time course-images of FM4-64 uptake at the hyphal tips. Hyphae stained by FM4-64 were examined by using fluorescence microscopy at different time points. The regions where fluorescence intensity was measured by ImageJ software were labeled by ellipse frame. Bars = 5 μm. (B) The bar chart shows the mean fluorescence intensity at the hyphal tip region. At least 15 hyphae of each strain were measured by applying ImageJ software at each time point. Error bars represent standard deviation (SD) and asterisks represent significant differences (P < 0.01). a.u., arbitrary unites. (C) F-actin network in appressoria (24 h) of Guy11 and ΔMoend3. Line-scan graphs show Lifeact:RFP fluorescence in a transverse section of individual appressorium. Bars = 5 μm. (D) F-actin in conidia of Guy11 and ΔMoend3. Bars = 10 μm. (E) Actin patches in hyphal tip regions. Bars = 10 μm.