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Micropatterned Coumarin Polyester Thin Films Direct Neurite Orientation
journal contribution
posted on 2014-11-26, 00:00 authored by Aleesha M. McCormick, Murthy V. S. N. Maddipatla, Shuojia Shi, Elaheh A. Chamsaz, Hiroshi Yokoyama, Abraham Joy, Nic D. LeipzigGuidance
and migration of cells in the nervous system is imperative for proper
development, maturation, and regeneration. In the peripheral nervous
system (PNS), it is challenging for axons to bridge critical-sized
injury defects to achieve repair and the central nervous system (CNS)
has a very limited ability to regenerate after injury because of its
innate injury response. The photoreactivity of the coumarin polyester
used in this study enables efficient micropatterning using a custom
digital micromirror device (DMD) and has been previously shown to
be biodegradable, making these thin films ideal for cell guidance
substrates with potential for future in vivo applications. With DMD,
we fabricated coumarin polyester thin films into 10 × 20 μm
and 15 × 50 μm micropatterns with depths ranging from 15
to 20 nm to enhance nervous system cell alignment. Adult primary neurons,
oligodendrocytes, and astrocytes were isolated from rat brain tissue
and seeded onto the polymer surfaces. After 24 h, cell type and neurite
alignment were analyzed using phase contrast and fluorescence imaging.
There was a significant difference (p < 0.0001)
in cell process distribution for both emergence angle (from the body
of the cell) and orientation angle (at the tip of the growth cone)
confirming alignment on patterned surfaces compared to control substrates
(unpatterned polymer and glass surfaces). The expected frequency distribution
for parallel alignment (≤15°) is 14% and the two micropatterned
groups ranged from 42 to 49% alignment for emergence and orientation
angle measurements, where the control groups range from 12 to 22%
for parallel alignment. Despite depths being 15 to 20 nm, cell processes
could sense these topographical changes and preferred to align to
certain features of the micropatterns like the plateau/channel interface.
As a result this initial study in utilizing these new DMD micropatterned
coumarin polyester thin films has proven beneficial as an axon guidance
platform for future nervous system regenerative strategies.
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coumarin polyesterMicropatterned Coumarin Polyester20 nmaxon guidance platformsystem regenerative strategiesorientation angle measurementsinjurycell guidance substratessurfacesystem cell alignmentfilmcell process distributionCNScontrol groups rangePNSDMD micropatterned coumarin polyesterrat brain tissue
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