Metal ion and buffer dependency of the covalent ligation of human telomere HT2

2011-12-31T00:01:36Z (GMT) by Jianying Qi Richard H. Shafer
<p><b>Copyright information:</b></p><p>Taken from "Covalent ligation studies on the human telomere quadruplex"</p><p>Nucleic Acids Research 2005;33(10):3185-3192.</p><p>Published online 2 Jun 2005</p><p>PMCID:PMC1142406.</p><p>© The Author 2005. Published by Oxford University Press. All rights reserved</p> Lane 1: Same as lane 1 in , top. Lane 2: Same as lane 4 in , top. Lanes 3–5: reactions were carried out in the same way as the one loaded in lane 4 in , top, except for replacing KCl with 20 mM NaCl (lane 3), 20 μM Pb (lane 4), 20 μM Ba (lane 5). Lanes 6–7: Same as lane 4 in , top, except for replacing MES with 10 mM Li-cacodylate (lane 6) or 10 mM Tris–HCl (lane 7).