jf5b01114_si_001.pdf (315.5 kB)
Metabolite Profiling of Barley Grain Subjected to Induced Drought Stress: Responses of Free Amino Acids in Differently Adapted Cultivars
journal contribution
posted on 2015-04-29, 00:00 authored by Alexandra Lanzinger, Thomas Frank, Gabriela Reichenberger, Markus Herz, Karl-Heinz EngelTo investigate cultivar-specific
metabolite changes upon drought
stress in barley grain, differently adapted cultivars were field-grown
under drought conditions using a rain-out shelter and under normal
weather conditions (2010–2012). The grain was subjected to
a gas chromatography–mass spectrometry-based metabolite profiling
approach allowing the analyses of a broad spectrum of lipophilic and
hydrophilic low molecular weight constituents. Multi- and univariate
analyses demonstrated that there are grain metabolites which were
significantly changed upon drought stress, either decreased or increased
in all cultivars. On the other hand, for proteinogenic free amino
acids increased concentrations were consistently observed in all seasons
only in cultivars for which no drought resistance/tolerance had been
described. Consistent decreases were seen only in the group of stress
tolerant/resistant cultivars. These cultivar-specific correlations
were particularly pronounced for branched-chain amino acids. The results
indicate that free amino acids may serve as potential markers for
cultivars differently adapted to drought stress.