ppat.1007477.g001.tif (2.09 MB)
Mapping RV-C binding domains in CDHR3.
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posted on 2018-12-10, 18:52 authored by Kelly Watters, Ann C. Palmenberg(A) Design of CDHR3 EC deletion constructs. (B) Cell lysates of HeLa cells transfected with these cDNAs were reacted with sucrose purified C15 virus (107 PFUe) as described in Methods. Western assays after immunoprecipitation with an α-His mAb detected CDHR3 (via α-FLAG) and captured virus (α-C15). Binding % is the observed C15 signal pixel count normalized to the CDHR3 protein (α-FLAG) signal in the panel above (Total Lab 100) relative to the WT CDHR3 lane (bold face).
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CDHR 3childhood virus-induced asthma susceptibilityEC 1 landing padY 529 formatCDHR 3 immunoprecipitationsRV-BCDHR 3 extracellular domains EC 1-3RV-Avirus infection VirusesRV-C interaction requirementsLDLtransduced cell lysatesCDHR 3 deletionsrhinovirus C speciesICAMcadherin-related family member 3rhinovirus C interaction3 extracellular N-linked modification sitesEC 1 domainamino-terminal EC 1 unit529YEC 3-mediated multimerization formatsY 529 variations
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