Macf1 localization and expression in <i>macf1a</i><sup><i>p1CH1</i></sup> <i>and macf1a</i><sup><i>p2CH1CH2</i></sup> <i>mutants</i>.

<p>A-C) Macf1a immunostaining (red) in WT and <i>macf1a</i><sup><i>p1CH1</i></sup><i>/macf1a</i><sup><i>sa12708</i></sup> <i>and macf1a</i><sup><i>p2CH1CH2</i></sup>. N≥ 3 ovaries, 20 oocytes <i>macf1a</i><sup><i>p1CH1</i></sup><i>/macf1a</i><sup><i>sa12708</i></sup> and 28 oocytes <i>macf1a</i><sup><i>p2CH1CH2</i></sup>. D) AV polarity in WT and lack of polarity in <i>macf1a</i><sup><i>sa12708</i></sup>, <i>macf1a</i><sup><i>p1CH1</i></sup><i>/macf1a</i><sup><i>sa12708</i></sup> and <i>macf1a</i><sup><i>p2CH1CH2</i></sup>/<i>macf1</i><sup><i>sa12708</i></sup> mutant eggs, where the cytoplasm (arrowheads) surrounds the yolk instead of forming the blastodisc. Mutant <i>macf1a</i><sup><i>p1CH1</i></sup> females produced an incompletely penetrant egg phenotype. Late blastula embryos from <i>macf1a</i><sup><i>p</i>3<i>PRD</i></sup><i>/ macf1</i><sup><i>sa12708</i></sup> and <i>macf1a</i><sup><i>p</i>3<i>PRD</i></sup> mutant females displayed normal AV polarity and development. Arrowheads in D indicate the egg cytoplasm. Scale bar: (A-C) 20 μm and (D) 100 μm.</p>