Macf1 localization and expression in macf1a^p1CH1 and macf1a^p2CH1CH2 mutants.

A-C) Macf1a immunostaining (red) in WT and macf1a^p1CH1/macf1a^sa12708 and macf1a^p2CH1CH2. N≥ 3 ovaries, 20 oocytes macf1a^p1CH1/macf1a^sa12708 and 28 oocytes macf1a^p2CH1CH2. D) AV polarity in WT and lack of polarity in macf1a^sa12708, macf1a^p1CH1/macf1a^sa12708 and macf1a^p2CH1CH2/macf1^sa12708 mutant eggs, where the cytoplasm (arrowheads) surrounds the yolk instead of forming the blastodisc. Mutant macf1a^p1CH1 females produced an incompletely penetrant egg phenotype. Late blastula embryos from macf1a^p3PRD/ macf1^sa12708 and macf1a^p3PRD mutant females displayed normal AV polarity and development. Arrowheads in D indicate the egg cytoplasm. Scale bar: (A-C) 20 μm and (D) 100 μm.