MOESM4 of Spontaneous and CRISPR/Cas9-induced mutation of the osmosensor histidine kinase of the canola pathogen Leptosphaeria maculans

Additional file 4: Fig. S2. Expression of the CRISPR components or mutation of hos1 does not impair pathogenicity on plants. Cotyledons of B. napus cv. Westar were inoculated with four strains, and lesions measured 14 days later. The wild type isolate D5 was sequentially transformed with the hos1 guide RNA construct (to create strain DV1) and the Cas9 construct (DV2), and then plated on iprodione to isolate a resistant strain (DV3).