MOESM2 of HIV-1 capsid uncoating initiates after the first strand transfer of reverse transcription

Additional file 2. Effect of FST mutations on capsid stability. (a-b) HeLa cells were infected with WT GFP-HIV VLP, or VLP carrying LTR mutations (LTR Del or LTR Chimera). Cells were lysed (a) 2 h or (b) 20 h.p.i. and cell lysate [input, I] was separated into soluble [S] and pellet [P] fractions by centrifugation through a sucrose cushion. CA was detected by immunoblotting. Blots are representative of 3 independent experiments.