13072_2017_163_MOESM2_ESM.pdf (1.04 MB)
MOESM2 of Functional dissection of Drosophila melanogaster SUUR protein influence on H3K27me3 profile
journal contribution
posted on 2017-12-01, 05:00 authored by Olga Posukh, Daniil Maksimov, Petr Laktionov, Dmitry Koryakov, Stepan BelyakinAdditional file 2: Figure S1. Comparison of ChIP results in SuUR mutants and in wild type obtained with H3K27me3 antibodies from different vendors and with the antibodies against H3K27me2. A—scatter plot of ChIP-chip signals obtained with the Abcam #6002 antibodies in SuUR mutants (abscissa) and in wild type (ordinate) [13]. B—scatter plot showing H3K27me3 ChIP-seq signals obtained with Cell Signaling Technology #9733 (CST #9733) antibodies in the same genotypes. C—the same analysis performed with Millipore #07-452 antibodies against H3K27me2. Datapoints inside 193 SSRs are shown in red. In both cases (A and B) H3K27me3 antibodies produce the characteristic skew (arrows): SSRs systematically show stronger signal in wild type strain as compared to SuUR mutants. This tendency is absent in case of H3K27me2 (C).