MOESM1 of User-friendly extraction and multistage tandem mass spectrometry based analysis of lipid-linked oligosaccharides in microalgae

Additional file 1. Multistage tandem mass spectrometry analyses of the structure of the LLO-released oligosaccharide isolated from C. reinhardtii XTB mutant by ESI-MSn. ESI-MSn spectra with n = 2 (panel A), n = 3 (panel B) and n = 3 (panel C) of permethylated Hex8HexNAc2 derivative (m/z 1107.6 corresponding to [M + 2Na]2+ precursor ion) isolated from C. reinhardtii XTB mutant. On each panel, the ion selected for the fragmentation analysis is shown with a diamond and its fragmentation pattern is proposed according to Prien et al. [40]. Black square: N-acetylglucosamine; grey circle: mannose, black circle: glucose. The fragment ions are labelled according to the nomenclature of Domon and Costello [41].