MOESM1 of Unbiased profiling of volatile organic compounds in the headspace of Allium plants using an in-tube extraction device

Additional file 1. Comparison of the peaks collected with ITEX and SPME devices. a. Total ion chromatograms (TICs) of VOCs in the HS of A. fistulosum (class01) obtained by ITEX-GC-TOF–MS (left) and SPME-GC-TOF–MS (right). b. VOC peak annotation with RI information obtained by ITEX-GC-TOF–MS (left) and SPME-GC-TOF–MS (right). c. The PCA score scatter plot of VOC profiles of A. fistulosum (class01) obtained by using ITEX-GC-TOF–MS and SPME-GC-TOF–MS. We used three biological replicates (six individual plants per replicate). AU arbitrary unit. Fluorobenzene, 4-bromofluorobenzene, and 1,2-dichlorobenzene-d 4 were used as ISs. Of these, peaks of luorobenzene and 4-bromofluorobenzene were detected (black arrow in Additional file 1).