MOESM1 of High cell density production of multimethyl-branched long-chain esters in Escherichia coli and determination of their physicochemical properties

Additional file 1: Figure S1. Protein expression in different E. coli RQ1 derivatives strains. A. Western blot analysis of RQ1 derivative strain expressing extra copies of S. coelicolor PCC complex. (1) RQ1/pMB07 induced with 0.1 mM IPTG. (2) RQ1/pMB07/pMB20 not induced. (3) RQ1/pMB07/pMB20 induced with 0.1 mM IPTG. B. Coomasie stained SDS-PAGE of RQ1/pMB07. (1) RQ1/pMB07 not induced. (2) RQ1/pMB07 induced with 0.1 mM IPTG. C. Western blot analysis of RQ1 derivative strain expressing extra copies of papA5. (1) RQ1/pMB07 induced with 0.1 mM IPTG. (2) RQ1/pMB07/pMB04 not induced. (3) RQ1/pMB07/pMB04 induced with 0.1 mM IPTG and 0.2 % l-Ara. D. Western blot analysis of RQ1 derivative strain expressing extra copies of fadD28. (1) RQ1/pMB07 induced with 0.1 mM IPTG. (2) RQ1/pMB07/pMB05 induced with 0.1 mM IPTG and 0.2 % l-Ara.