MOESM1 of Combination of nanoparticle-based therapeutic vaccination and transient ablation of regulatory T cells enhances anti-viral immunity during chronic retroviral infection

Additional file 1: Figure S1. A) Schematic illustration of functionalized multi shell calcium phosphate (CaP) nanoparticles. B) C57BL/6 mice were chronically infected with FV (>6 weeks) and therapeutically vaccinated either with PBS or functionalized CaP nanoparticles. 7 or 14 days post vaccination (d.p.v.), mice were sacrificed for analyzes. C) The percentage of CD4+ CD43+ and CD8+ CD43+ effector cells is shown. D) Frequency of granzyme B (GzmB) expressing CD43+ CD8+ T cells and CD43+ CD8+ FV-specific T cells identified by tetramer staining recognizing the H-2Db-restricted FV gag epitope is depicted. E) 7 and 14 d.p.v., mice were sacrificed, and infectious centers in the spleen were determined. The figure summarizes the results of 3 independent experiments. Statistical analysis was performed by student’s t-test. *p<0.05; **p<0.005.